Yes and No. If you remove the second disk in slot 2 and reboot, you get a red error e16 flashing which means disk not found. If you go to the web access, it says disk 2 is disconnected. I tried reloading the firmware and restarting, but there doesn't seem to be any way to clear the error 16. Although the cloudstor is fully functional with disk one, but it looks like you have to live with the red flashing e16 forever after.The manual says to use the remove disk button in the web access, but there is no such thing. Hopefully someone from Buffalo is reading this and will recreate the remove function. Does any one know a work around?