On a fat32 parition, you can't have afile that is larger than 4GB. On a Fat32 partition, to even have a 4GBfile, which would be the largest file you could have, you would have tohave a fat32 partition larger than 4GB. OS+FAT+the file.
If I recall correctly the largest fat32 partition you can have is 2TB.
FAT32 has a 2 Terabyte Partition Limit and a 4 Gigabyte File-Size Limit.